ABSTRACT
It has been clearly documented that Elaegnaceae have a variety of medicinal uses including anti-ulcerogenic activity. We therefore hypothesized that Elaeagnus angustifulia L. [E. A.] fruit might be involved in the control of stimulated gastric acid secretion. To address this question, we investigated the pharmacological effect of the E. A. fruit extract on carbachol-induced acid secretion in a pylorus-ligated rat. In this study we used pylorus ligation method. Briefly, animals were anesthetized and two cannulas were introduced into the stomach through esophagus to inject drug and pylorododenal junction to collect the stomach juice. Carbachol or histamine was infused into jugular vein and gastric juice was collected in 10-min periods to titrate with NaOH 0.01 N. Our results showed that the E. A. fruit extract dose and time dependently decreased the carbachol- [but not histamine-] stimulated gastric acid secretion when it administered at increased steady level [at 30 minute] of carbachole time-course curve. Stimulated acid secretion was completely suppressed at a dose 600 mg/kg 30 min after drug administration and this inhibitory effect persisted up to the end of experiments [100 min]. Using E.A. fruit extract simultaneously with carbachol infusion had no effect on acid secretion. These results suggested that E.A. fruit has a pH-dependent anti-secretory action on cholinergic-stimulated gastric acid secretion by intragastric administration. It is likely that the inhibitory effect is mediated by cholinergic nervous system and/or non-neuronal membranes